To developed an educational tool for an online OSCE to evaluate the practice readiness of the fourth-year nursing students. Online professional proficiency evaluation of nursing students can be a valid alternative to traditional methods. We designed a one group pre-posttest study. The 51 nursing students were recruited. We implemented a 3-stage of online Objective Structured Clinical Examination Midwifery Test Package (OSCEMTP). The package covered seven segments which developed to enhance the cognitive capability, clinical capability, and professional capabilities. The Practice readiness questionnaire was developed to assess the nursing students' readiness. Content validity index was 1, and reliability with Cronbach's alpha was 0.79. OSCEMTP contains 7 segments: 1) contraceptive; 2) antenatal care; 3) admission interview; 4) first stage of labor; 5) second and third stage of labor; 6) fourth stage of labor and 7) breastfeeding. Each segment has a scenario, a competency evaluation, and tools box for demonstration of clinical skills. Practice readiness was improved in the post-test (p<0.001). Performance OSCEMTP should be evaluated across diverse population of nursing students to ascertain its validity. An online OSCE Test package is an education tool to enhance the practice readiness before turning to be a professional nurse. [For the full proceedings, see ED652228.]