Kangaroo care (KC) or kangaroo mother care (KMC), sometimes called skin-to-skin contact, is a technique of newborn care where babies are kept chest-to-chest and skin-to-skin with a parent. The research was carried out experimentally to investigate the effect of the early kangaroo care by using Neonatal Comfort Behavior Scale in invasive interventions applied to the babies during early kangaroo care who born as healthy normal birth. Eighty babies who born at Adana Maternity and Child Health Hospital delivery room between 1 July and 31 October 2016 and their mothers constituted the research sampling. The babies were inserted into the experimental (n = 40) and control groups (n = 40) by the simple random sampling method. The invasive interventions were executed to the babies included in experimental group during early kangaroo care and COMFORTneo Behavior Scale was applied. The crying times of babies in both groups were measured with the aid of stopwatch. The level of comfort of the group receiving kangaroo care was found to be higher than those who did not. Significant connection was confirmed statistically positive, and highly between total score and duration of crying (r = 0.925; p = 0.000). It was determined that crying time increases and comfort decreases with increment in total score. As a result, it was found that the early kangaroo care applied during invasive interventions of newborns is an effective method in increasing baby's comfort who born at delivery room.