The aim of the quantitative study is to identify the digital literacy levels of prospective teachers in terms of several variables. The sample consisted of 354 prospective teachers studying in different departments of Sakarya University College of Education. The 30-item instrument used to gather the data was the "Digital Literacy Scale" developed and used by the researchers. The scale was composed of 5 different factors namely information literacy, visual literacy, software literacy, technology literacy and computer literacy. Exploratory Factor Analysis, Cronbach alpha, t-test and Anova were used for data analysis. Results showed that in terms of gender variable digital literacy levels of male prospective teachers and in terms of department variable digital literacy levels of computer education and instructional technology teaching department were found high. Besides, the digital literacy levels of prospective teachers having continuous Internet connection or a computer that they can continuously use were found high. In addition, the research found that prospective teachers' personal income levels had no effect on their digital literacy levels.