By hosting a college students' laboratory skills contest, we provide an interesting activity for students to cultivate good laboratory practices and thereby improve their laboratory skills. The substance of this contest is the detection of the hardness of water, which involves numerous laboratory skills such as solution preparation, weight by…

Heterocyclic compounds are an integral part of research, particularly in the pharmaceutical field. The synthesis of such compounds in an undergraduate laboratory can help students understand the relevance and importance of their field of study. Our experimental activity introduces a novel procedure to synthesize coumarin, a heterocyclic formed by…

An experiment for the synthesis of N-acyl derivatives of natural amino acids has been developed as part of the Distributed Drug Discovery (D3) program. Students use solid-phase synthesis techniques to complete a three-step, combinatorial synthesis of six products, which are analyzed using LC-MS and NMR spectroscopy. This protocol is suitable for…

Chemometric techniques such as partial least-squares (PLS) regression have been applied with huge success to a wide array of chemical problems including multicomponent analysis of analytes in complex mixtures. Despite this, there are few examples of laboratory pedagogical exercises that involve students in the acquisition of chemical data from…

A laboratory experiment is described that extracts the tetrapyrrolic teal-colored biliverdin IXa, as its dimethyl ester, from commercially available emu eggshells. The extraction of ~10 mg samples of biliverdin is simple and requires two 3 h lab periods: A two-step acid digestion and liquid-liquid extraction, followed by short silica gel flash…

The laboratory protocol presented here takes about 3 hours to perform and investigates protein and lipid interactions. Students first purify His6-tagged human apolipoprotein A-I (apoA-I) with Ni-NTA affinity resin in a simple batch protocol and prepare multilamellar vesicles (MLV) from pre-dried phospholipid films. When apoA-I is added to the MLV,…

Traditional developmental biology laboratory classes have utilized a number of different model organisms to allow students to be exposed to diverse biological phenomena in developing organisms. This traditional approach has mainly focused on the diverse morphological and anatomical descriptions of the developing organisms. However, modern…

Benzoyl peroxide is used as a bleaching agent for flour and whey processing, a polymerization initiator in the synthesis of plastics, and the active component of acne medication. Because of its simplicity and wide application, benzoyl peroxide is a target molecule of interest. It can be affordably synthesized in three steps from bromobenzene using…

This study aims to examine students' reasoning based on the model in each laboratory work style and find the effect on scientific attitude and student activity. This study uses a quantitative approach with the experimental method. The study was conducted in three classes with different lab work styles. Arduino and sensors were used as a…

A single fly RT-PCR protocol has recently been developed to detect the presence of the persistent, horizontally transmitted Nora virus in "Drosophila." Wild-caught flies from Ohio were tested for the presence of the virus, with nearly one-fifth testing positive. The investigation presented can serve as an ideal project for biology…

Many biology educators at the undergraduate level are revamping their laboratory curricula to incorporate inquiry-based research experiences so that students can directly participate in the process of science and improve their scientific reasoning skills. Slugs are an ideal organism for use in such a student-directed, hypothesis-driven experience.…

Research-based learning motivates students to identify with different subjects. The orientation program MINTgrün at Technische Universität Berlin offers two study semesters for open choices of teaching modules, including a series of specially designed laboratories covering topics like robotics, construction, environmental research, programming,…

The molecular basis of evolution is an important and challenging concept for students to understand. In a previous article, we provided some of the scientific background necessary to teach this topic. This article features a series of laboratory activities demonstrating that molecular events can alter the genomes of organisms. These activities are…

We present a practical activity focusing on two main goals: to give learners the opportunity to experience how the scientific method works and to increase their knowledge about enzymes in everyday situations. The exercise consists of determining the amylase activity of commercial detergents. The methodology is based on a qualitative assay using a…

Plastics are more prevalent in our society than ever before, yet the general public has a limited understanding of why plastics have properties that are vastly different from other common materials such as glass and ceramics. This lab is designed to introduce students to several introductory principles of polymer science and their relation to the…