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Schlamadinger, Diana E.; Kats, Dina I.; Kim, Judy E. – Journal of Chemical Education, 2010
Laboratory experiments that focus on protein folding provide excellent opportunities for undergraduate students to learn important topics in the expanding interdisciplinary field of biophysics. Here, we describe the use of Stern-Volmer plots to determine the extent of solvent accessibility of the single tryptophan residue (trp-59) in unfolded and…
Descriptors: Thermodynamics, Chemistry, Biophysics, Laboratory Experiments
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Sanchez, Katheryn M.; Schlamadinger, Diana E.; Gable, Jonathan E.; Kim, Judy E. – Journal of Chemical Education, 2008
Protein folding is an exploding area of research in biophysics and physical chemistry. Here, we describe the integration of several techniques, including absorption spectroscopy, fluorescence spectroscopy, and Forster resonance energy transfer (FRET) measurements, to probe important topics in protein folding. Cytochrome c is used as a model…
Descriptors: Spectroscopy, Physics, Chemistry, Biophysics