Enzyme-like catalysis is the use of artificial catalysts to catalyze chemical reactions, which has the characteristics of enzymatic catalysis, such as high selectivity, high efficiency, and mild reaction conditions. In recent years, 2D materials, thickness ranging from a single to several atomic layers, have become a hot research topic in the…

The discipline-based education research (DBER) report from the National Research Council (2012) highlighted the existence of a research-practice gap in science instruction in higher education and the need to identify strategies to close it. This study hypothesizes that one potential factor is instructors' access to research-based…

Describes an undergraduate biochemical experiment involving the isolation and characterization of acid phosphatase from readily available materials. (Author/SL)

Describes a laboratory experiment demonstrating the aldehyde oxidase activity in wild type and mutant fruit flies using a quantitative assay. The experiment can be performed in one hour. (MA)

Discusses the kinetic behavior associated with the interaction of metal ions with apocarbonic anhydrase, focusing on the formation of two metallocarbonic anhydrase--the biochemically active Co(II) and the inactive Ni(II)derivatives. (GA)

Describes an undergraduate biological chemistry laboratory experiment which provides students with an example of pseudo-first-order kinetics with the cardiac glycoside inhibition of mammalism sodium and potassium transport. (SL)

This experiment can be used as either a demonstration or a laboratory activity illustrating the principles of affinity chromatography to purify an enzyme. (BB)

Describes a convenient, inexpensive experiment in enzyme kinetics developed for the undergraduate biochemistry laboratory at the University of Virginia. Required are a single beam visible spectrophotometer with output to a recorder, a constant temperature, a commercially available enzyme, substrates, and buffers. (BT)

This inexpensive but informative experiment for undergraduate biochemistry students involves isolating phosphatidyl choline from spinach leaves. Emphasis is on introducing students to techniques of lipid extraction, separation of lipids, identification using thin layer chromatography, and identification of fatty acids. Three periods of three hours…

Described are several laboratory investigations that may be used to introduce biochemistry. Topics covered include carbohydrates, lipids, proteins, and metabolism. Reactions, stock solution formulas, and procedures are listed. Emphasized are individualizing work, designing alternative investigations, solving problems, and drawing conclusions. (KR)

Describes a biochemistry experiment involving the kinetics of passive diffusion of solutes across artificial membranes formed by dispersing phospholipids in aqueous medium. (MLH)

Describes an experiment in which the reaction rate and equilibrium constant are obtained for the fumarase-catalyzed interconversion of fumaric acid and L-malic acid. (MLH)

This demonstration uses the electrophoresis of indicators to demonstrate the principles involved in the separation of isoenzymes. (BB)

Describes a set of biochemistry laboratory experiments in which tetrameric hemoglobin is separated into its subunits then formed again by recombination of the subunits. (MLH)