An experiment was developed for a first year food chemistry laboratory course that demonstrates the influence of several aspects of polysacharide structure on the rate of amylase catalyzed hydrolysis. The experiment would be particularly well-suited to a first year chemistry course that was targeted at nonchemistry majors, such as health related…

Changes in environmental conditions can have a profound impact on organismal processes. For aquatic organisms, changes in dissolved oxygen concentration can have major repercussions, notably for physiological processes that have high long-term energetic costs and are more likely to rely on aerobic metabolic pathways. Here we propose a simple…

This article describes a ten week long laboratory exercise designed for undergraduate students aimed at enhancing their understanding of physiological and biochemical responses of plants under dehydration stress. Hypothesis was built around previous reports which suggested that exogenous application of certain elicitors leads to transient…

Electrocardiography (ECG) is one of the most widely used methods in clinical diagnosis. Here we describe an experimental approach that offers hands-on learning of its basic principles. An experimental model that consists of a rubber foil with a low electrical conductivity and a DC power unit is used to simulate the body and the electric dipole of…

A simple bioassay was designed for the detection of adenosine, a human metabolite involved in several physiological processes, on the basis of the functionalization of gold nanoparticles with DNA oligonucleotides selectively binding adenosine. The test was possible thanks to the selective and sensitive binding of adenosine by two DNA…

In the present article, we describe a 3-day experimental workshop on glycemia regulation and type 1 diabetes that engages students in open-ended investigations and guided experiments leading to results that are not already known to them. After an initial questioning phase during which students observe PowerPoint slides depicting the glycemia…

An educational device was created to develop a hands-on activity to illustrate how atherosclerosis can dramatically reduce blood flow in human vessels. The device was conceived, designed, and built at the University of Coimbra, in response to a request from the Exploratorio Infante D. Henrique Science Centre Museum, where it is presently…

Hands-on activities with live organisms allow students to actively explore scientific investigation. Here, I present activities that combine guided inquiry with direct instruction and relate how nutrition affects the physiology and behavior of the common housefly. These experiments encourage student involvement in the formulation of experimental…

Evolutionary history and structural considerations constrain all aspects of animal physiology. Constraints on invertebrate locomotion are especially straightforward for students to observe and understand. In this exercise, students use spiders to investigate the concepts of adaptation, structure-function relationships, and trade-offs. Students…

We have realized that our Biology undergraduate students learn biological concepts as established truths without awareness of the body of experimental evidence supporting the emerging models as usually presented in handbooks and texts in general. Therefore, we have implemented a laboratory practice in our course of Physiology and Biophysics, aimed…

The polygraph (lie detector) may be an ideal vehicle for converting aspects of a physiology course into an interdisciplinary course, enabling discussion of interrelationships between science, technology and society (STS) and social dilemmas arising from technological developments. The authors present a way of incorporating the polygraph into a…

Describes a laboratory experience for upper-level science students that provides a hands-on approach to understanding the basics of experimental physiology. Students design an experiment to determine the relative importance of dilution of plasma proteins in the overall renal excretory response following volume expansion with intravenous saline.…

Because of the daily impact of biotechnology, it is important that students have some knowledge and experience with biotechnology in order to enable them to deal with the issues that arise as a result of its implementation. The purpose of this workbook is to assist in the efforts to expose students to the concepts of biotechnology through hands-on…