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Using "Pseudomonas Putida xylE" Gene to Teach Molecular Cloning Techniques for Undergraduates
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Dong, Xu; Xin, Yi; Ye, Li; Ma, Yufang – Biochemistry and Molecular Biology Education, 2009
We have developed and implemented a serial experiment in molecular cloning laboratory course for undergraduate students majored in biotechnology. "Pseudomonas putida xylE" gene, encoding catechol 2, 3-dioxygenase, was manipulated to learn molecular biology techniques. The integration of cloning, expression, and enzyme assay gave students…
Descriptors: Undergraduate Students, Biotechnology, Molecular Biology, Science Experiments