This research aims at investigating the readiness level of pre-service biology teacher to follow Blended Collaborative Problem Based Learning (BCPBL) model class. This research also aims at investigating the differences in the readiness level of pre-service biology teachers in the BCPBL class due to the semesters differences. The participants were randomly selected from the students in semester 2, 4, 6, and 8 of Biology Education Study Program in Indonesia. From the total population of 750 students, 27 students, 24 students, 29 students, and 21 students were selected from the 2nd, 4th, 6th, and 8th semester students respectively. The instrument used in this research was the questionnaire modified from the one developed by Xiong et al. (2015). The reliability of the questionnaire had been tested and obtained a reliability index of 0.893. The data obtained were analyzed descriptively and followed by using one-way analysis of variance (ANOVA). Based on the results of this research it is concluded that in general pre-service biology teacher have a high level of readiness to follow the BCPBL class. Semester differences do not cause significant differences in the level of students' readiness to follow BCPBL model.