The aim of this survey was to determine whether there is a relationship between prosocial tendencies and the level of emotional intelligence in a sample of teachers. The partial goals were to determine whether there is a difference in the score of emotional intelligence and prosocial tendencies between male and female teachers. The research group consisted of 997 primary and secondary school teachers, of whom 109 were men (average age 44.9 years) and 889 women (average age 45.17 years). The Czech translation of the Trait Emotional Intelligence Questionnaire - Short form, TEIQue-SF was used to determine the emotional intelligence score. The second method applied in the research is the abbreviated form of the Prosocial Personality Battery (PSB). The correlation coefficient calculation shows a significant negative relationship between the level of emotional intelligence and age, and the length of work experience. This result was found both within the group as a whole and for the individual groups of men and women.