The purpose of this study was to analyze faculty selections of course content in introductory-adapted physical education courses syllabi pertaining to content on inclusion at three universities in Chile. In this content analysis, course syllabi from three different Chilean physical education teacher education programs were analyzed. From the course syllabi, we analyzed: (a) selected content, (b) disability-specific content, (c) evaluations selected, and (d) bibliographical sources. Overall, the findings of this content analysis reveal several approaches that might influence the professional preparation of future physical educators in teaching students with disabilities in Chilean schools. We concluded the need to increase course content and critical discussions devoted to inclusion in physical education in Chile. Additionally, there is a need to incorporate the views and opinions of persons with disabilities to inform future content that is relevant and appropriate.