Previous educational articles on microfluidic technology mainly focused on continuous microfluidics, so we set up an undergraduate laboratory experiment to introduce droplet microfluidics to students. Students can understand the concept of microfluidic channels and the principles of droplet microfluidics, prepare polydimethylsiloxane (PDMS) devices and split-flow strategy, explore the controllable preparation and monodispersity of chitosan (CS) microspheres, and gain a more comprehensive understanding of microfluidic technology. The experiment has the advantages of easy operation and a short cycle, and it is suitable as an undergraduate experiment.