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Direct linkZhang, Wenqiang; Cao, Yibin; Xu, Lishan; Gong, Jufang; Sun, Meihao – Biochemistry and Molecular Biology Education, 2015
Gel filtration chromatography (GFC) separates molecules according to size and is one of the most widely used methods for protein purification. Here, red fluorescent protein (RFP), green fluorescent protein (GFP), yellow fluorescent protein (YFP), cyan fluorescent protein (CFP), and/or their fusion proteins were prokaryotically expressed, purified,…
Descriptors: Biochemistry, Science Instruction, Molecular Structure, Light
Dong, Xu; Xin, Yi; Ye, Li; Ma, Yufang – Biochemistry and Molecular Biology Education, 2009
We have developed and implemented a serial experiment in molecular cloning laboratory course for undergraduate students majored in biotechnology. "Pseudomonas putida xylE" gene, encoding catechol 2, 3-dioxygenase, was manipulated to learn molecular biology techniques. The integration of cloning, expression, and enzyme assay gave students…
Descriptors: Undergraduate Students, Biotechnology, Molecular Biology, Science Experiments
