Biology learning needs to support sustainable development. This research was a quantitative-qualitative sequential explanatory mixed research. This research aimed to obtain information about the relationship between naturalist intelligence and environmental responsibility behavior. This research was conducted in February-August 2019. The quantitative research data were obtained by using a questionnaire, and the qualitative research data were obtained by doing interviews and observations. The data hypothesis was tested using a correlation test with the Product Moment Pearson formula. The result of this research showed that there was a positive relationship between naturalist intelligence and environmentally responsible behavior with regress equation Y[with circumflex] = 20.331 + 0,7524X, Correlation coefficient (r) = 0.66, and determination coefficient (r[superscript 2]) = 43.56%. it indicated that the interpretation value was strong. r[superscript 2] value means that 43.56% environmentally responsible behavior correlates by naturalist intelligence, and other factors influence 56.44% environmentally responsible behavior. Biology learning must provide innovations related to increasing students' knowledge about the environment. This study concludes that there is a correlation between the two variables.