Wheat powdery mildew is an important fungal pathogen of wheat with an obligatory biotrophic lifestyle (a parasite that can only develop on a living host). We investigated the genetics of this host-pathogen interaction by using phenotyping and PCR assays to detect genes in both wheat and powdery mildew, which are known determinants of the outcome…

It has been shown that active learning strategies are effective in teaching complex STEM concepts. In this study, we developed and implemented a laboratory experiment for teaching the concepts of Boolean logic gates, molecular beacon probes, molecular computing, DNA logic gates, microRNA, and molecular diagnosis of hepatocellular carcinoma, which…

Racial biases, which harm marginalized and excluded communities, may be combatted by clarifying misconceptions about race during biology lessons. We developed a human genetics laboratory activity that challenges the misconception that race is biological (biological essentialism). We assessed the relationship between this activity and student…

The CRISPR/Cas9 system is a powerful tool for gene editing and it has become increasingly important for biology students to understand this emerging technique. Most CRISPR laboratory teaching modules use complex metazoan systems or mammalian cell culture which can be expensive. Here, we present a lab module that engages students in learning the…

The COVID-19 pandemic has forced the Bioinformatics course to switch from on-site teaching to remote learning. This shift has prompted a change in teaching methods and laboratory activities. Students need to have a basic understanding of DNA sequences and how to analyze them using custom scripts. To facilitate learning, we have modified the course…

We used a novel approach that combined direct PCR of plant tissue and SRAP DNA fingerprinting to seek to identify the crosses made in "Brassica rapa" used in the Wisconsin Fast Plants. Our goal was to develop a rapid method for students to use in a biology, botany, or genetics class to identify the parental types (purple stem, non-purple…

Laboratory practical sessions are critical to scientific training in biology but usually fail to promote logical and hypothesis-driven reasoning and rely heavily on the teacher's instructions. This paper describes a 2-day laboratory practicum in which students prepare and analyze larval cuticle preparations of "Drosophila" segmentation…

The MicRoboCop project introduces chemistry students to the concepts and protocols of synthetic biology. MicRoboCop is a biosensor that has been designed for unique applications in forensic chemistry. The lab is carried out in collaboration with an instructor teaching a genetics course. Students in the biology course synthesize biosensors using…

Most undergraduate students do not have practical experience working with model organisms despite having theoretical knowledge of their importance. This practical was developed to offer students experience working with three species of nematode, including "Caenorhabditis elegans." In the first task, students prepare slides of three…

We provide a protocol for rapid DNA extraction from spiders suitable for undergraduate practical sessions. Students who were previously naïve to the theory and laboratory technique of DNA barcoding were successfully able to extract and recover 29 DNA sequences from 16 species of small spiders in the family Linyphiidae. We anticipate that with…

Programmed cell death, or apoptosis, is a cellular pathway by which individual cells self-destruct for the benefit of the organism. In this practical paper, we describe laboratory exercises with an inquiry-based learning (IBL) approach in which undergraduate students compared apoptosis among different types of cultured cells. Ultraviolet (UV)…

Recent years have seen a dramatic increase in our understanding of the social behaviour of microbes. Here, we take advantage of these developments to present an undergraduate laboratory exercise that uses the cooperative flocculating behaviour of yeast ("Saccharomyces sp.") to introduce the concept of inclusive fitness and teach the…

We developed labs on the evolution of antibiotic resistance to assess the costs and benefits of replacing traditional laboratory exercises in an introductory biology course for majors with a course-based undergraduate research experience (CURE). To assess whether participating in the CURE imposed a cost in terms of exam performance, we implemented…

All the cells of higher organisms have the same DNA but not the same proteins. Each type of specialised cell that forms a tissue has its own pattern of gene expression and, consequently, it contains a particular set of proteins that determine its function. Here, we describe a laboratory exercise addressed to undergraduate students that aims to…

Course-based undergraduate research experiences (CUREs) can engage large numbers of students and provide a structured environment in which to learn valuable research skills. The ability to implement laboratory-based CUREs was hindered by the COVID-19 pandemic, generating a greater need for online options. A pilot study of an adaptation of a fully…