The threat of climate change makes it increasingly important for biologists and the public to understand how organisms respond to temperature. The Metabolic Theory of Ecology (MTE) predicts that temperature should affect organism performance, with implications for species interactions and ecosystems that span disciplines including mathematics,…

Newton's law of cooling describes an object whose temperature decreases exponentially with time. Because of its many applications, it is a frequent topic of introductory physics labs. In this article, I describe an experiment designed for the freshman year algebra-based physics course that applies this law to answering the question, "Why…

While metabolism is a fundamental process in physiology, the measurement of metabolism by living animals often requires specialized and expensive equipment. In 2016, I developed a novel laboratory exercise for animal physiology students where students were able to observe the effects of temperature and long-term temperature acclimation on the…

Laboratory practical sessions are critical to scientific training in biology but usually fail to promote logical and hypothesis-driven reasoning and rely heavily on the teacher's instructions. This paper describes a 2-day laboratory practicum in which students prepare and analyze larval cuticle preparations of "Drosophila" segmentation…

In this teaching laboratory, the students are directed in an exercise that involves designing and performing an experiment to determine estrogen's role in regulating delayed implantation (diapause) in female rats. To encourage active participation by the students, a discussion question is provided before the laboratory exercise in which each…

This research aims to analyse the progression of 13-14 year-old students' ideas regarding the concept of energy transfer by means of heat in three biological mechanisms of thermal regulation. The outputs of 270 students, grouped in 77 cooperative groups, are analysed by comparing their explanations before and after conducting an experimental…

Most undergraduate students do not have practical experience working with model organisms despite having theoretical knowledge of their importance. This practical was developed to offer students experience working with three species of nematode, including "Caenorhabditis elegans." In the first task, students prepare slides of three…

Governance and regulation of the use of live animals in research and teaching is examined in Australia, Malaysia, Indonesia, the Philippines, Singapore, Thailand, China, Japan and India. A comparison of the systems in different countries will enable the determination of best practice and fit-forpurpose regulation. The most comprehensive government…

We provide a protocol for rapid DNA extraction from spiders suitable for undergraduate practical sessions. Students who were previously naïve to the theory and laboratory technique of DNA barcoding were successfully able to extract and recover 29 DNA sequences from 16 species of small spiders in the family Linyphiidae. We anticipate that with…

Programmed cell death, or apoptosis, is a cellular pathway by which individual cells self-destruct for the benefit of the organism. In this practical paper, we describe laboratory exercises with an inquiry-based learning (IBL) approach in which undergraduate students compared apoptosis among different types of cultured cells. Ultraviolet (UV)…

Background: The goals of laboratory experiences include developing knowledge base, research skills, and scientific communication abilities. Objective: The aim was to assess an inquiry-based laboratory activity using the model organism "Caenorhabditis elegans" in relation to learning goals. Method: Students in a "Biopsychology"…

A laboratory experiment is described that extracts the tetrapyrrolic teal-colored biliverdin IXa, as its dimethyl ester, from commercially available emu eggshells. The extraction of ~10 mg samples of biliverdin is simple and requires two 3 h lab periods: A two-step acid digestion and liquid-liquid extraction, followed by short silica gel flash…

All the cells of higher organisms have the same DNA but not the same proteins. Each type of specialised cell that forms a tissue has its own pattern of gene expression and, consequently, it contains a particular set of proteins that determine its function. Here, we describe a laboratory exercise addressed to undergraduate students that aims to…

The California Blackworm ("Lumbriculus variegatus") is a freshwater segmented worm species that has been used by biology instructors as a model system for inquiry-based student investigations. The blackworm dorsal blood vessel pulsation rate is easily quantified. Moreover, this species can facilitate the study of neuromuscular…

Science popularisation festivals attract more and more people every year, showing that there is a need to satisfy public curiosity. Such events are good occasions to disseminate knowledge to a large audience. The results of scientific research bring advances for mankind. Since research mostly depends on public funds, it is important to gain…