This guidance is written to assist State educational agencies (SEAs), local educational agencies (LEAs), and schools in understanding and implementing preschool programs supported with funds under Title I, Part A (Title I) of the Elementary and Secondary Education Act of 1965, as amended (ESEA). Although each SEA may consider this guidance in the development of its own guidelines and standards, SEAs are free to identify alternative approaches that are consistent with applicable Federal statutes and regulations. Guidance in this document replaces previous non-regulatory Title I guidance on serving preschool children. It addresses Title I requirements in the ESEA and answers questions raised by SEAs, LEAs, and other officials regarding using Title I funds to serve preschool children. Recipients of Title I funds may refer to this guidance when administering or operating preschool programs supported with Title I funds. Selected Resources to Support High-Quality Early Learning Programs are appended.