The goal of the present study was to investigate preschool teachers' emotion socialization responses to Turkish preschoolers' emotional expressions based on children's age and gender. The participants in the current study were 12 preschool full time teachers from 4 preschool and 288 preschoolers ranging in age from 4 to 6 years in Aksaray. In order to perform naturalistic observation a coding system was created by researcher based on previous studies. The results indicated that preschool teachers more likely encouraged children to show empathy to others, teach positive emotions, encouraged expressing positive emotions, matched positive emotions, responded physical affection in 4 year old age children more than 5 and 6 year old age children. Preschool teachers were more often minimized negative responses, distracted, labeled negative emotion in 4 year old age children than 5 and 6 year old children. Results also revealed that there was a significant but low relationship between children's gender and teacher's responses to negative emotional expressions and a modest relationship between children's gender and teacher's responses to positive emotional expressions.