Electrophoresis is integral to analytical and biochemistry experiences in undergraduate education; however, fundamental principles of the method are often taught in upper-level laboratories through hands-on experiences. A laboratory activity is reported that teaches the concepts of electrophoretic mobility and electroosmotic flow. A single…

The artificial intelligence chatbot ChatGPT was used to answer questions from final exams administered in two general chemistry courses, including questions with closed-response format and with open-response format. For closed-response questions, ChatGPT was very capable at identifying the concept even when the question included a great deal of…

High-performance liquid chromatography (HPLC) experiments are an important part of the undergraduate analytical instrumentation laboratory courses and usually use commercial C18 silica-packed reversed-phase columns. In this experiment, a simple method of preparing SiO[subscript 2] core-shell packed columns was developed to enable students to…

High school students and students in the first years of college have difficulties in understanding the topic of stoichiometry and, in particular, the concept of limiting reactant. Proposals aimed at overcoming these learning obstacles tend to focus on analogies, and there are few sequences that approach teaching from an experimental perspective.…

Although diffusion is the subject of beginning chemistry classes, there are a variety of experiments, such as the spread of perfume in the classroom, that are often "incorrectly" explained by diffusion alone. To eliminate this common misconception, a recently published method is modified to determine the diffusion constant of hydrogen…

Photo-oxidation is one of the main causes of oxidative deterioration in food. Understanding of the mechanisms of photo-oxidation is essential for food science students when pursuing careers in food safety and quality assurance. However, few food chemistry laboratories emphasize photo-oxidation. Thus, we develop a laboratory activity that trains…

Organizing undergraduates in chemistry, energy engineering, and chemical engineering specialties to participate in an energy transformation experiment can encourage them to think about the impact of human activities on nature and stimulate them to learn more about the topic. This laboratory experiment demonstrates a general approach to the…

An experiment for the undergraduate physical chemistry laboratory is described in which the Raman spectrum of liquid "para"-difluorobenzene is recorded and assigned. A density functional theory (DFT) calculation of the 30 normal modes of the molecule is undertaken using computational chemistry software. Students use group theory to…

Recently, two-dimensional (2D) nanostructures, such as graphene and few-layer MoS[subscript 2], have been applied to wide fields based on their intriguing performances, and they contribute to developments of clean energy, biomedicine, and environmental protection. The synthesis of 2D nanostructures involves chemistry, materials, and…

Metabolism is a challenging subject for bioscience students due to the intrinsic complexity of the metabolic network, as well as that of the overlapping mechanisms of metabolic regulation. Collaborative learning based on a problem-based learning approach can help students to successfully learn and understand metabolism. In the present article, we…

This work describes the design and testing of a low-cost light-emitting diode (LED)-based nephelometer for turbidity analysis. It also describes the use of this nephelometer for context-based learning (CBL) experiments designed for teaching laboratories. The LED nephelometer is simple, low-cost, and robust, providing similar results as a…

Apurinic/apyrimidinic (AP or abasic) sites are deoxyribonucleic acid (DNA) lesions that result from the loss of a nucleobase by hydrolysis of the "N"-glycosyl bond. AP sites are the most frequent lesions in cells and are caused by environmental and cancer therapeutic genotoxins. Herein, the bicinchoninic colorimetric assay, typically…

Successful demonstration of lipid assembly chemistry to a general audience can be challenging on both physical (nanoscopic dimensions of lipid assemblies) and intellectual (complex, interconnected concepts) accounts. Quite often, an instructor has to leave key concepts out of what is being offered due to time constraints, which unfortunately…

NMR spectroscopy is a powerful spectroscopic tool that not only allows the determination of structures after synthesis, as usually explained to students of Organic Chemistry, but also is useful in many other fields such as medicine imaging, real-time industrial processes, material sciences, or metabolomics. In this experiment, students performed a…

A comprehensive laboratory work that allows students to gain hands-on skills in energy-efficient mechanochemistry is described. Standard university curricula are paying very little attention to mechanochemistry, although skills in this discipline are highly demanded among chemistry graduates. In this study, a comprehensive description of…