This article describes a ten week long laboratory exercise designed for undergraduate students aimed at enhancing their understanding of physiological and biochemical responses of plants under dehydration stress. Hypothesis was built around previous reports which suggested that exogenous application of certain elicitors leads to transient…

While metabolism is a fundamental process in physiology, the measurement of metabolism by living animals often requires specialized and expensive equipment. In 2016, I developed a novel laboratory exercise for animal physiology students where students were able to observe the effects of temperature and long-term temperature acclimation on the…

Regulation is a key concept for understanding the dynamics of metabolism in bacteria. This report outlines a simple laboratory experiment aimed at studying a key form of regulation in bacterial metabolism, exemplifying how microbes switch the flux of carbon towards alternative metabolic fates as a function of nutrient availability and according to…

Flow cytometry has become an important tool in the fields of veterinary, medicine, and biological sciences in general. However, in the introductory and even in advanced university degree courses, students do not have many opportunities to be in contact with this type of technology. This situation motivated us to design a simple and inexpensive…

There have been growing calls for inquiry, a practice-oriented way of engaging students in authentic scientific work, yet research has shown some challenges that might impede inquiry practices in educational settings. In this study, we proposed an approach that blends educational gaming with physical experiments. We developed an educational game,…

The method for producing monoclonal antibodies (MAbs) was first published in 1975. MAbs have revolutionised research, diagnosis, and disease treatment approaches. While students need a good understanding of MAb production, teaching the key steps via traditional methods can be challenging. Numerous resources exist, but these focus on hybridoma…

This research aims to analyse the progression of 13-14 year-old students' ideas regarding the concept of energy transfer by means of heat in three biological mechanisms of thermal regulation. The outputs of 270 students, grouped in 77 cooperative groups, are analysed by comparing their explanations before and after conducting an experimental…

The monitoring of pH changes due to dissolved carbon dioxide from algal balls offers a way for students to acquire good understanding of the processes of photosynthesis and respiration. Here, 54 high school participants were enlisted to conduct the experiment physically and with an Augmented Reality (AR) tool developed. A survey conducted after…

The development and evaluation of a two-session laboratory class, based on Geneswitch technology and sex determination in the fruit fly Drosophila melanogaster, is described. Geneswitch system allows conditional control of gene expression. A laboratory exercise has been devised for sophomores in order to illustrate how Geneswitch technology can be…

Most undergraduate students do not have practical experience working with model organisms despite having theoretical knowledge of their importance. This practical was developed to offer students experience working with three species of nematode, including "Caenorhabditis elegans." In the first task, students prepare slides of three…

In recent years the possibility of observing by microscopy the dynamic activity of living cells has been largely pursued. We have developed a low-cost (~ 260 euros) on-stage cell incubator for inverted optical microscopes. This device allows to keep cells in good conditions for their survival and proliferation. The device is based on the use of…

This study examines the epistemic disciplinary knowledge that students consider when addressing a real food emergency that requires their engagement in inquiry and argumentation practices. The study comprised two phases: (1) designing an experiment to study the emergency and (2) evaluating the real outcome of the emergency. The participants were…

Research and education are two areas often compartmentalised or separated by an abyss, linked in this study by a service-learning methodology, the Small World Initiative project. The programme instruction starts at the university, reaches secondary school and returns to university to complete the research, challenging young students to discover…

We provide a protocol for rapid DNA extraction from spiders suitable for undergraduate practical sessions. Students who were previously naïve to the theory and laboratory technique of DNA barcoding were successfully able to extract and recover 29 DNA sequences from 16 species of small spiders in the family Linyphiidae. We anticipate that with…

Integrating genetic engineering and molecular biology lab activities into open inquiry-based teaching is an innovative, unique process that has taken place in Israeli high schools since 2018, becoming part of biology studies for 11th- and 12th-grade students. Engaging these students in lab techniques and practices in this field enables them to…