There are numerous research studies that have investigated the attitude toward women managers across the globe. Most of the findings indicate that positive and negative attitudes toward women managers can be influenced, among other things, by cultural variables. The goal of this study was to determine if there are differences in the attitude of graduating business students in comparison to those at an earlier stage of their studies. Samples of second and third year students were compared to those of graduating seniors in the College of Business of a regional university in the southeastern part of the United States using the Women As Managers (WAMS) questionnaire. The expectation was that going through the educational process at the university level may support more positive attitude toward women as managers. The ANOVA and MANOVA analyses indicate that there are no statistically significant differences in the attitude toward women managers at the undergraduate level. Consistent with previous research, however, we found significant differences in attitude toward women managers by gender. The sample of female students, regardless of their educational level, had more positive attitude than their male counterparts toward women managers.