Providing both introductory information and biosecurity protocols in laboratory, farm, and field settings is central to student learning and safety. However, even when clear protocols are provided, students do not fully understand the consequences of their actions. We present a crime scene that requires evidence investigation to improve basic skills and inquiry to identify biosecurity breaches. The crime-scene format engages students and encourages critical thinking about the negative effects of actions when working in various environments. This approach not only improves student skills through forensic microscopy but advances student retention of biosecurity requirements.