The emergence of digitalisation focusing on media literacy is becoming more prevalent and thus forcing educators to embrace innovations as our digital natives are now shifting their attention to digital technologies. This study aimed to develop and measure an assessment instrument for media literacy focusing on knowledge, skills, and values for digital natives in Malaysia. Adopting the digital intelligence (DQ) framework, we have constructed the assessment instrument relevant to our digital natives of secondary school students and distributed it across schools in eleven states and one Federal Territory of Malaysia. We received a complete set of instruments from 1,276 respondents of Form Two and Form Four students and analyse the data by using descriptive and statistical analysis through SPSS. The findings of the study contribute to a more holistic assessment instrument that recognises the increasingly complex areas of knowledge, values, and skills for digital natives living in diverse, digitally-mediated environments.