The importance of university students' electronic health (eHealth) literacy has been established in the literature, yet the association with computer skills is absent. In this study, a total of 5,672 university students were recruited from Xinyang University. Data were collected from an online questionnaire, including the Chinese eHealth Literacy Scale (C-eHEALS), online health information and a computer skills questionnaire. Pearson's correlation coefficient and t-tests were used in the analysis. Of the 5,672 university students, 72.36% were female, 93.20% were aged 18-22 years. The total C-eHEALS score was 26.75 ± 5.86 (mean ± SD), and there was a significant difference in the total score between groups with low-level and high- level eHealth literacy (P < 0.001). The most popular three methods for accessing health information among both groups were using search engine (P = 0.035), mobile phone APP (P < 0.001) and face-to-face inquiry (P = 0.697). Students with eHealth literacy used a greater variety of eHealth resources than students with low eHealth literacy to access eHealth information. Pearson's correlation coefficient between C-eHEALS and computer skills was 0.621 (P < 0.001). A positive correlation exists between the eHealth literacy and computer skills of university students. However, both eHealth literacy and computer skills are poor, and targeted improvement is urgently needed.