Enzymes are nature's catalysts, mediating chemical processes in living systems. The study of enzyme function and mechanism includes defining the maximum catalytic rate and affinity for substrate/s (among other factors), referred to as enzyme kinetics. Enzyme kinetics is a staple of biochemistry curricula and other disciplines, from molecular and…

This article seeks to provide researchers and practitioners in laboratory education, particularly those involved in the curriculum design and implementation of teaching laboratories at university level, with a conceptual framework and a working model for an integrated assessment of learning domains, by attending to a more holistic approach to…

An introductory guide to deducing the mechanism of chemical reactions is presented. Following a typical workflow for probing reaction mechanism, the guide introduces a wide range of kinetic and mechanistic tools. In addition to serving as a broad introduction to mechanistic analysis for students and researchers, the guide has also been used by…

The problem of obtaining the apparent equation of motion and shape of a moving body from its arbitrary given equation of motion in special relativity is considered. Also the inverse problem of obtaining the body's equation of motion from a known equation of motion of its image is discussed. Some examples of this problem solution are considered. As…

This article explores the practical relevance of pragmatism as a research paradigm through the example of a piece of pragmatic research that not only used both quantitative and qualitative research methods but also exploited the inherent duality of the data analyzed. Thus, the article aims to make the case that pragmatism as a research paradigm…

In recent years, national policy experts have questioned the overall quality of educational research, and they have suggested that researchers strengthen their scientific methods by maximizing the use of experimental designs. To promote more rigorous methodology, we discuss several new and often-overlooked opportunities for incorporating…

The Prime Minister's Committee on Natural Science in Education in Great Britain reported in 1918 that "...in many schools more time is spent in laboratory work than the results obtained can justify." Seventy years later this conclusion can often still be drawn. This is particularly a problem in open distance education where laboratory practicals,…

Describes two new exercises in classical conditioning that use earthworms and houseflies. The animals are available year-round and pose no risk to the students or instructor. The conditioned stimuli are odorants. These elicit a conditioned response of contraction in worms or proboscis extension in flies. (MJP)

Describes the Tardigrada as a group of animals suitable for close study in project work. Gives reasons for their suitability and an illustrated identification key. Discusses possible investigations into the ecology and physiology of tardigrades. (Author/CW)

Discusses who has the right to determine the use of animals in scientific research which includes medical experimentation and the toxicity testing of compounds, ranging from pesticides to cosmetics. Provides a list of questions for consideration and discussion. (RT)

Describes the necking of polymer sheets that occurs when they are stretched. Discusses the effect and the mechanisms of necking. Lists four references. (YP)

Discusses the role of dogs in research, considering their use in studies related to: behavior; aging; anesthesia; gastrointestinal surgery; the brain; organ transplants; radiobiology; trauma and shock; arterial diseases; hemophelia; ophthalmology; diabetes; nutrition; cancer; lupus; cyclic neutropenia; thyroiditis; hepatitis; skeletal system,…

Discusses issues and the public's concerns related to using laboratory animals for research purposes. Indicates that several perceptions (such as recognizing that the public's attitudes toward respect for animal life are no more irrational than many broader attitudes toward the sacredness of life) characterize the most responsible of recent…

The Grignard reagent used in the laboratory synthesis of organic compounds is the product resulting from the reaction of an alkyl or aryl halide with elemental magnesium. Describes the structure, formation, and some reactions of the reagent. (YP)

Reports an experiment for examining protein-ligand binding using the enzyme carbonic anhydrase. Describes the experimental procedures consisting of the syntheses of an azosulfonamide and its complex formation with carbonic anhydrase. (YP)