In this "Optimizing AI in Higher Education: SUNY FACT[superscript 2] Guide, Second Edition," you will find updated information to reflect the changing landscape of generative AI technology. We offer practical suggestions for how to help educators and students achieve AI literacy and find the right methods of using or eschewing generative AI in their pedagogy. The second edition represents both an update to the first Guide, but also a recognition of the fact that generative AI is a tool in constant development; we will continue to advocate for equitable education and access for all students and educators as this dynamic and innovative field continues to grow. The book results from a Task Group charged by the FACT2 Council with Judith Littlejohn as Chair. Co-chairs Billie Franchini and Jeffrey Riman, who also served as editors, led the Task Group's year-long efforts.