Background: Sexually transmitted diseases (STDs) remain a significant public health concern as more than 2.5 million infected in 2021, necessitating effective educational interventions to promote knowledge and safe practices. The study analyzes secondary data using SPSS. The dataset includes pre- and post-interventional data of KISS (Knocking out Infections through Safer-sex and Screening) intervention, which includes Sexually transmitted infections (STIs) knowledge assessment scale score among a group of military personnel and their spouses at an army base in Washington. Result: The study found that STIs knowledge increased from 15 to 25 (66.6%) on a scale of 30 after the intervention. Paired sample t test indicates a significant increase in knowledge scores following intervention (p<0.01). After intervention, 92.4% participants were more likely to practice safe sex. In addition, the intervention found to be positively correlated (Pearson correlation, p < 0.05) in their responses. Almost all participants were confident talking about STIs (97%), comfortable with their group (97%), comfortable asking questions (95.5%), were willing to get screened regularly for STIs (94%), and to recommend to a friend (88%). Thematic analysis revealed 40% participants decided to practice safe sex whereas about 16% of respondents expressed the intention to ask the doctor to perform regular screening tests. None of the demographic variables showed a significant association with the knowledge assessment scores (p > 0.05). Conclusion: The intervention KISS found effective in improving knowledge and behavior of STIs prevention along with high participant acceptance suggests its potential for broader implementation to diverse populations and settings with a control group on a larger scale. [For the full proceedings, see ED656038.]