During the preschool period, children's interest in reading increases, and their attitudes toward books develop with the means and opportunities provided by their teachers. Teachers' attitudes develop during their undergraduate education as pre-service teachers. This study aims to reveal preschool pre-service teachers' attitudes toward children's literature and reading habits and the relationship between these attitudes. The relational screening model was used, and the study was conducted with 343 preschool pre-service teachers. The data were collected through the "Preservice Teacher's Attitude toward Children's Literature Scale (ACLS)" and "Pre-service Teacher's Attitudes toward Reading Habit Scale (ARHS)." The descriptive, difference and correlation analyses were used to analyze the collected data. As a result of the study, the attitudes of preschool pre-service teachers toward children's literature were found to be high, and their attitudes toward reading habits were moderate. Regarding the differences in the attitudes according to the variables, the following results were obtained: female pre-service teachers' attitudes toward children's literature and reading habits were significantly higher than males; attitudes of 4th-grade pre-service teachers' toward children's literature were significantly higher than others; regarding the attitudes toward reading habits, the only significant difference is in the "attitude toward the development of reading habit" sub-dimension, in favor 1st-grade pre-service teachers; attitudes toward children's literature showed a statistically significant difference in favor of pre-service teachers who took children's literature course, but taking this course did not affect their attitudes toward reading habits. A positive and weak relationship was found between preschool pre-service teachers' attitudes toward children's literature and reading habits.