Objective: This research was conducted to determine the relationships between psychological well-being, social support, and work-life balance and the mediating effect of psychological well-being on the relationship between social support and work-life balance. Methods: Data were collected from a convenience sample of 277 participants. Data analysis was done with regression and the Pearson correlation coefficient. Regression-based mediation analysis developed by Hayes (2013) was used to examine the mediation effect of psychological wellbeing. Results: Work-life balance is negatively related to social support (r=-0.51) and, positively related to psychological well-being (r=0.50). As a result of the mediation analysis based on regression to determine the mediating effects, it was concluded that psychological well-being had a mediating effect on the relationship between social support and work-life balance. The bootstrapp (10000) method was used to determine whether the mediation analysis was significant. Conclusions: These findings suggest that the inverse relationship between social support and work-life balance is at least partly explained by the level of psychological well-being.