The study aims to examine the effect of the level of intra-organizational entrepreneurship in higher education institutions on the level of organizational innovation and to determine whether the perceived organizational development level has a mediating role in this effect. In the research, it is examined whether the academicians' perceptions of intra-organizational entrepreneurship, organizational innovation, and organizational development differ significantly according to demographical and professional variables. It also examines the subject in depth according to the views of academicians qualitatively in accordance with the results of the quantitative research. The research is carried out with an explanatory sequential design, one of the mixed design methods. The correlational survey, causal comparison, and Structural Equation Modeling (SEM) are used to test the theoretical relationships empirically. The qualitative part of the research is conducted according to the phenomenological design. The quantitative sample of the research is a total of 501 faculty members having various academical degrees selected from foundation and state universities in the 2020-2021 academic calendar through proportional stratified sampling. The qualitative study group of the research is 15 academicians who were selected by using purposive sampling among the participants who constitute the quantitative sample group. As a result of the research, while the average scores of the Organizational Entrepreneurship Scale were found to be high, the Organizational Innovation Scale and the Organizational Development Level Determination Scale mean scores were found to be moderate. When all the findings were evaluated together, it is found that the academics' perceptions of intra-organizational entrepreneurship in higher education institutions indirectly affect organizational innovativeness through their perceptions of organizational development.