This study aimed to explore the relationship between basic psychological needs, motivational regulations, and self-esteem in Turkish high school physical education environment. Nine hundred and fifty seven high school students (505 girls, 452 boys) were applied the questionnaire pack in physical education lessons. Students' general self-esteem, basic psychological needs and motivational regulations toward physical education were assessed. Hierarchical multiple regression analysis results revealed that Turkish high school students' autonomy, competence, and relatedness need satisfaction in physical education positively predicted students' global self-esteem. Physical education teachers were recommended to consider creating need supportive lesson environment for adolescents to enhance their optimal psychological functioning and well-being.