The purpose of this study was to determine the managerial and cultural values adopted by managers working in universities and whether these values differ according to gender, age, professional experience, managerial experience, and their faculties. This study was conducted as a survey. The participants of this study were composed of 100 deans, vice deans, and heads of departments in Afyon Kocatepe University. Study data were collected using the Managerial and Cultural Values Adopted by the Managers Scale developed by Turan, Durceylan and Sisman (2005). This study showed that university managers adopted feminine values more commonly, were closer to a collectivist value dimension, the power distance was higher, and ambiguity avoidance was lower.