This study is focused on the analysis of online intercultural competence from a gender perspective. It uses online communications and student self-perceptions to explore similarities and differences. Videoconferencing was used as a tool for communication in higher education. A quantitative and qualitative methodologies were applied, including quantifying content analysis. The sample consisted of pairs of university students. The results show very similar scores between men and women in communication and self-perception in affective aspects. Statistical differences were found in the behavioural communication and in cognitive self-perception. Communications of negative nature are scarce in both groups. This study helps to understand the similarities and differences in students' communication and self-perception in an intercultural online context.