Purpose: Our purpose was to gather and evaluate accurate, up-to-date information on physical education (PE) policy implementation across multiple U.S. states and regions. Methods: A U.S. Physical Education and Physical Activity Policy questionnaire was developed and completed by 4,845 public-school PE teachers from 25 U.S. states. The U.S. Physical Education and Physical Activity Policy assessed PE quantity (days per week and minutes per week), class sizes by grade level, and practices that limit PE access. Descriptive statistics and correlations of PE minutes per week with class size were calculated in aggregate and individually by state. Results: Aggregate PE quantities in elementary, middle school, high school, and for students with disabilities were well below the recommendations (150 min/week elementary and 225 min/week secondary) and varied between states. Average students/class varied by state, and significant positive correlations between weekly PE minutes and students/PE class were observed. Discussion: PE access is limited across the United States, and stronger commitment to PE policy and policy implementation is needed.