E-learning tools technology is a paradigm shift in facilitating learning in our world today and the driving force of education in developed countries. The main purpose of this study is to investigate the accessibility and utilization of E-learning tools for teaching biology in secondary schools in Ilorin metropolis. The study was guided by the technology acceptance model theory, and employed a descriptive survey design. The population of the study were all biology teachers in public secondary schools in Ilorin metropolis. The sample size was determined from the estimate of the target population which is 368. The sample size for this study was 250 respondents from 100 public secondary schools. A well-structured questionnaire was the instrument. The data collected was analysed using descriptive and inferential statistics. The findings showed lack of access and low or non-utilization of E-learning tools for teaching biology in public secondary schools in Ilorin metropolis while factors observed to be responsible for the findings are low or lack of awareness on the part of teachers, poor policy formulation to incorporate the use of e-learning tools and poor infrastructure in the schools.