The purpose of this study was to compare selected motor fitness profile of football referees in Cross River and Akwa Ibom States. Motor fitness profiles compared were running speed and agility of the referees. Standardized equipment and procedures were employed in the tests. To achieve the objectives of the study, two research hypotheses were raised to serve as a guide to the study. The quasi-experimental research design was adopted for the study. A total of twenty (20) subjects were selected using stratify random sampling technique. Data collected were analyzed using descriptive and inferential statistics. The descriptive statistics were the mean, range and standard deviation while the inferential statistic was the independent "t" test employed to test for significant difference between the mean scores of subjects from the two states. The level of significance was set at 0.05 with 18 degrees of freedom. Findings of the study revealed that referees from Cross River and Akwa Ibom States were similar in running speed and agility when compared. Based on these findings of the study, appropriate conclusions and recommendations were made.