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Discovery of "Escherichia coli" CRISPR Sequences in an Undergraduate Laboratory
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Militello, Kevin T.; Lazatin, Justine C. – Biochemistry and Molecular Biology Education, 2017
Clustered regularly interspaced short palindromic repeats (CRISPRs) represent a novel type of adaptive immune system found in eubacteria and archaebacteria. CRISPRs have recently generated a lot of attention due to their unique ability to catalog foreign nucleic acids, their ability to destroy foreign nucleic acids in a mechanism that shares some…
Descriptors: Undergraduate Students, Biochemistry, Molecular Biology, Science Laboratories
Two-Dimensional Nuclear Magnetic Resonance Structure Determination Module for Introductory Biochemistry: Synthesis and Structural Characterization of Lyso-Glycerophospholipids
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Garrett, Teresa A.; Rose, Rebecca L.; Bell, Sidney M. – Biochemistry and Molecular Biology Education, 2013
In this laboratory module, introductory biochemistry students are exposed to two-dimensional [superscript 1]H-nuclear magnetic resonance of glycerophospholipids (GPLs). Working in groups of three, students enzymatically synthesized and purified a variety of 2-acyl lyso GPLs. The structure of the 2-acyl lyso GPL was verified using [superscript…
Descriptors: Diagnostic Tests, Science Laboratories, Biochemistry, College Science