Although, the study of the relationship between the University and entrepreneurship has been on the increase, special attention should be given to the subject of University entrepreneurship. In this regard, despite studies have emerged that have tried to differentiate the entrepreneur from who is not, the characterization of this type of entrepreneur is not yet clear. An entrepreneur is an individual that combines, at the same time, University studies with the generation and management of his/her own business. The variables that can help describe the characteristics of this type of entrepreneur can be academic stress, internal locus of control and risk taking propensity. This study used a quantitative, descriptive-comparative and correlational method. Through a sample of 356 University students from Mexico and Bolivia, it was possible to find significant differences between University entrepreneurs (n = 154) and non-entrepreneur students (n = 202). In addition, the results show that entrepreneurs have a lower level of academic stress compared to non-entrepreneurial students, which can be explained by the first group having high levels of internal locus of control and risk taking propensity. This demonstrates the need to study University entrepreneur from his/her own context using a particular approach.