This study aims to: Produce teaching materials in the form of microbiology e-books based on research results on the isolation of styrofoam degrading soil bacteria. Testing the effect of using e-books on improving students' science process skills. The e-book preparation method refers to the Lee and Owens development model and is implemented in learning with the PBL model in the experimental and control groups. The results showed that: e-book material feasibility, e-book teaching materials and e-book practicality with very valid criteria. E-books are proven effective in improving students' science process skills with high criteria, so e-books are suitable for use as teaching materials to support learning. Can be used to solve problems that may be encountered in everyday life.