Measurement of mental rotation presents a serious challenge to cognitive researchers owing to the lack of a single comprehensive measure that can be applied across the developing age groups. Objective of the present study was to develop and validate a new measure of mental rotation for preadolescent and adolescent age groups. Items were conceptualized and constructed based on existing theories. Study I checked the suitability of these items among preadolescent and adolescent age groups. After revisions, Study II was done to evaluate the item properties using item response theory. Subsequently done Confirmatory Factor Analysis provided evidence for the construct validity of the new measure. Finally, Study III was done to develop the age wise and gender wise norms for preadolescent and adolescent age groups. The newly developed measure was found to have sufficient reliability and validity and hence can be widely applied for measuring mental rotation of preadolescents and adolescents.