Using the Intercultural Sensitivity Scale formulated by G. M. Chen and Starosta, the intercultural sensitivity of English major students, non-English major students, and multinational employees in China were investigated. In this study, intercultural sensitivity was defined as consisting of interaction engagement, interaction confidence, respect for cultural differences, interaction enjoyment, and interaction attentiveness. The focus of this research was to explore how intercultural sensitivity was structured among subjects of different English learning and intercultural communication experience. A series of results generated by multiple regressions showed different weightings of the 5 elements of intercultural sensitivity of the 3 subsamples. For example, respect for cultural differences for English major students was affected by interaction engagement and enjoyment, whereas for multinational employees it was only affected by interaction enjoyment. The study also found that English major students felt interaction enjoyment more easily than non-English major students and multinational employees. (Contains 5 tables.)