Job satisfaction among teachers is important for their motivation to effect changes. However, in China, job satisfaction in early childhood education is a pressing issue, which leads to high attrition rates among preschool teachers. Thus, this study examined the role of school culture regarding job satisfaction and teaching autonomy among teachers as a potential mediator of this relationship in the preschool settings in China. Using survey data from 597 participants and by conducting structural equation modeling, this study found that school culture exerted a direct and significant impact on job satisfaction among teachers, whereas curriculum autonomy mediated the relationships in this regard. The results indicate the need for additional in-depth studies of the job satisfaction of preschool teachers in China, which exhibits a hierarchical structure.