Purpose: The aim of this study is to investigate whether human auditory frequency tuning can be influenced by tonal language experience. Method: Perceptual tuning measured via psychophysical tuning curves and cochlear tuning derived via stimulus-frequency otoacoustic emission suppression tuning curves in 14 native speakers of a tonal language (Mandarin) were compared to those of 14 native speakers of a nontonal language (English) at 1 and 4 kHz. Results: Group comparisons of both psychophysical tuning curves (p = 0.046) and stimulus-frequency otoacoustic emission suppression tuning curves (p = 0.007) in the 4-kHz region indicated sharper frequency tuning in the Mandarin-speaking group relative to the English-speaking group. The auditory tuning was better at the higher (4 kHz) than the lower (1 kHz) probe frequencies (p < 0.001). Conclusions: The sharper auditory tuning in the 4-kHz cochlear region is associated with long-term tonal language (i.e., Mandarin) experience. Experience-dependent plasticity of tonal language may occur before the sound signal reaches central neural stages, as peripheral as the cochlea.