In our pronunciation courses, segmental level in English pronunciation plays a very important role in perception and teaching of English pronunciation. This research aims to investigate into the most salient pronunciation problems at segmental level by fresh college students from Mainland China through an experimental study, and it is found that the mismatches identified in the segmental level of English pronunciation are corresponded with the phonological features of the utterance of Chinese English. Moreover, in order to improve fresh college students' pronunciation and achieve the pronunciation of Standard English, some pedagogical implications and suggestions about teaching strategies are provided in this study which aimed at the most salient mismatches discovered in the research findings.