Objective: In this study, we investigate university students to learn about their barriers to improving and/or maintaining a healthy lifestyle and their needs and interests regarding the development of a tailored intervention to promote physical activity and a healthy diet. Participants: The qualitative research included three focus groups consisting of 22 university students. Methods: The interview grid was developed around the variables of the ecological Conceptual Framework of the Quebec Ministry of Health and Social Services. Data were analyzed using NVivo software. Results: Time constraints due to a heavy workload, high costs and low motivation were the reasons students failed to participate in physical activity and/or adopt a healthy diet. Furthermore, they perceived that on-campus food and physical activity are more expensive and the quality of food is poorer. Conclusions: Students are nevertheless interested in the development of a tailored intervention to promote physical activity and a healthy diet.