The reliability and validity of the PLAY"fun" and PLAY"basic" tools were assessed. The PLAY"fun", PLAY"basic", Canadian Agility and Movement Skill Assessment (CAMSA) obstacle course, and Physical Activity Questionnaire for Children (PAQ-C) were administered to children (aged 8-14 years) in two remote Canadian communities. Inter-rater reliability of the PLAY"fun" and PLAY"basic" were good-to-excellent for average measures and moderate-to-good for single measures. The PLAY"fun" subscales were poor-to-excellent for average and single measures. The internal consistency of the PLAY"fun" tool was good, PLAY"basic" was poor-to-good, and the individual subscales were poor-to-good. Convergent validity was moderate-to-large for the PLAY tools and CAMSA obstacle course, and small-to-moderate for the PLAY tools and the PAQ-C, and small-to-moderate for the PLAY tools and age. For children and youth in northern Canada, the motor competence aspect of physical literacy is most accurately assessed using the complete PLAY"fun" tool and two raters.