In this paper, data obtained from a university website accessibility and usability validation process are analyzed and used to demonstrate how the design process can affect the online experience for users with disabilities. Interviews, observations, and use data (e.g. where users clicked on a page or what path taken through a site) were collected. Findings indicate that using automated validation tools does not necessarily ensure complete accessibility. Students with low vision found many of the pages hard to use even though automated validation did not indicate issues for visual disabilities. While the pages were accessible for blind users, low vision students who did not use specialized software had access problems. Findings from this study are used to present principles for web designers interested in creating and testing usable and accessible websites. (Contains 4 tables, 3 figures, and 10 footnotes.)