The main aim of our study was to adapt the Questionnaire on Teacher Interaction (QTI) to an Estonian context. The QTI was translated and evaluated by three educational researchers and validated with a sample of 508 students from grades 6 and 9 in 13 middle schools. When statistical analyses were utilized to investigate reliability and validity of the EstQTI, validity was supported to some degree. However, the underlying circumplex model was not fully confirmed and, therefore, further improvement of the instrument is needed. The factors in the study explained a variance of 68% and the reliability of the eight resulting scales ranged between 0.75 and 0.92. The results are encouraging and indicate that EstQTI has the potential to investigate teacher-student interaction in Estonia.