Raman scattering spectroscopies are important analytical tools in modern chemistry. Here, we describe the construction of the unamplified Raman scattering analysis (URSA) instrument: an inexpensive, open-table Raman spectrometer that uses a 532 nm diode-pumped laser as a light source. Upon application of this spectrometer to DNA extracted from…

Despite the growing importance of agricultural microbiology at the applied, productive and ecological levels, undergraduate degrees in Agronomy generally do not feature much microbiological experimentation. To address this deficiency, we have designed an experimental programme that evaluates the potential of plant growth-promoting rhizobacteria…

Plant blindness is becoming a universal phenomenon among both students and adults. They do not know the names of the plants in their immediate environment, nor are they interested in familiarising themselves with the plants that surround them. Teachers and outdoor education can play a vital role in reversing and preventing this phenomenon;…

Critical analysis (CA) skills are crucial in higher education and should be incorporated into the curriculum at that level. The unique characteristics of abstract biology materials and the involvement of prospective Biology teachers (PBTs) in scientific investigation activities make them highly relevant for teaching CA skills. Inquiry activities…

Metacognition is often described as the awareness and regulation of learning. It uses strategies which include monitoring one's own thinking, engaging in active planning and self-evaluating one's study habits. Bloom's taxonomy can be used as a metacognitive tool to guide students' study strategies and thus improve their academic performance by…

In the past few decades, chemistry has evolved to interact with various disciplines to synergistically help tackle global challenges. This, in turn, requires that newer generations of chemistry students are trained to be more flexible in accepting and coordinating new concepts. In this experiment, pineapple was used as a key model to allow for the…

Plant awareness disparity (PAD, formerly plant blindness) is the tendency not to notice plants in one's environment, which leads to the perspective that plants are unimportant. In this paper, we explore how plants and animals are represented in introductory biology textbooks to determine if these texts are contributing to PAD in undergraduate…

Empowerment of creative thinking skills is important in every lecture activity, including practicum. This study aimed to analyze students' creative thinking skills in Plant Microtechnique practicum. This research used descriptive method in which the participants were the seventh semester biology education students (n=20) of State University of…

The CRISPR/Cas9 system is widely used for editing genes in various organisms and is a very useful tool due to its versatility, simplicity, and efficiency. To teach its principles to post-graduate students we designed a laboratory activity to obtain and analyze PDS3 mutants in "Arabidopsis thaliana" plants consisting of: 1) Design of…

The use of red cabbage ("Brassica oleracea var. capitata F. rubra") extract as a pH indicator is a versatile and popular demonstration that is employed by educators in many schools and universities. Previous variations of this demonstration have used red cabbage extracts to prepare paper that changes color when solutions of acid or base…

The time-resolved detection of very low intensity light emission has become an essential capability in many areas of science including molecular biology, fluorimetry, DNA sequencing, virus detection, nanoparticle research, and optical materials development. Among the most basic techniques for the detection of rapidly fluctuating low-intensity…

Drought is a major environmental stress factor that affects the growth and development of plants. All plants have to maintain the reactive oxygen species within certain levels for normal cellular homeostasis by means of their antioxidant systems, which can be classified as enzymatic and non-enzymatic. Plants under drought stress generate an excess…

We used a novel approach that combined direct PCR of plant tissue and SRAP DNA fingerprinting to seek to identify the crosses made in "Brassica rapa" used in the Wisconsin Fast Plants. Our goal was to develop a rapid method for students to use in a biology, botany, or genetics class to identify the parental types (purple stem, non-purple…

The COVID-19 pandemic has highlighted the need to adapt to and rebound from unexpected change and uncertainty. The increasing climate chaos of the Anthropocene additionally underscores the necessity of resilient societies and individuals. Individuals able to problem-solve in emergent situations are integral to a resilient society, and science…

Agricultural teacher education programs are designed to prepare competent teachers who are ready to teach students in public schools. One aspect of agricultural teacher education is ensuring teachers are ready to lead instruction in various aspects of school-based agricultural education (SBAE), such as teaching students various technical…