A mismatch between furniture and body characteristics can cause musculoskeletal discomfort during prolonged sitting for a variety of learners. The purpose of this study was to explore university students' perceived changes in pain and/or discomfort, perceived level of comfort, and perceived ability to focus and learn while using four common desk chair styles. After recording standing and seated height, sixty-seven university students completed a survey tool which contained six sections: (1) demographics, (2) type of desk chair used and time in desk chair, (3) pain and/or discomfort rating and location prior to and after desk chair use, (4) impact of desk chair on pain and/or discomfort, (5) rating of desk chair comfort, and (6) rating of desk chair adjustability. A desk chair with padding and a large frame was rated significantly highest in overall comfort. Participants reported significantly increased upper back pain when using a desk chair with minimal arm support. During periods of prolonged seating, desk chairs should accommodate various sized bodies and have cushioning, as well as support for the upper body.